Nui nā ʻāpana cell ma hope o ka mechanical lysis o ka cell suspension.Pehea e wehe ai i kēia mau ʻāpana?E nānā kākou i nā ala like ʻole:

1. E hoʻohana i ke ʻano dilution.Ke hoʻoheheʻe ʻia nā cell, hiki iā lākou ke hoʻonui, e lilo lākou i mea nui a ʻoi aku ka nui, a me ka liʻiliʻi o ka ʻōpala o ka cell.
2. Aia kekahi noho maoli.ʻOi aku ka wikiwiki o ka noho ʻana o nā kelepona ma mua o ka hapa nui o nā ʻāpana: : E hoʻoneʻe i ka hoʻokuʻu kelepona i loko o kapaipu centrifuge, a i ka wā e pohō ai ka hapa nui o nā pūnaewele, hiki ke omo ʻia ka hopena o luna, a laila hoʻohui ʻia i ka solution moʻomeheu e hoʻopaneʻe ai i nā cell.Hiki ke hoʻohana pinepine ʻia kēia ʻano hana, ʻoiai i kēlā me kēia manawa hiki iā ʻoe ke nānā inā kūpono ia i kāu mau koi ma lalo o ka microscope.
3. Haʻahaʻa-wikiwiki centrifugehiki ke wehe i nā ʻōpala, maʻamau 700g, 5mins
4. Ke centrifuging, Ma lalo o ke kūlana i lawa ka helu o nā pūnaewele, e ho'ēmi i ka manawa centrifugation, e like me 5min, 1000rpm, ma kahi o 3min, 1000rpm, a wehe i ka supernatant, no ka mea,ʻo ka necrosis a me ka'ōpala ka mea maʻamau i ka supernatant!Pono e hana ʻia kēia hana i hoʻokahi wale nō i ka wā o ka holoi ʻana ma mua o ka incubation!

ʻOiai he nui nā ala e wehe ai i nā ʻōpala cell, e ʻike ʻoe ʻo ka centrifugation kekahi o nā ala ʻoi loa!

A no ka mea, ʻo nā ʻiʻo a me nā cell mea kanu nā pā hale i haku ʻia me ka cellulose, hemicellulose a me ka pectin, pono pono e wili i ke one quartz a i ʻole ka pauka aniani me ka hoʻonā hoʻoneʻe kūpono a i ʻole mālama ʻia me cellulase e hoʻokō i ke kumu.ʻOi aku ka paʻakikī o ka hoʻokaʻawale ʻana o ka cell bacterial, no ka mea, ʻo ka ʻiʻo o ka pā o ka pā bacterial he mea paʻa covalent o peptidoglycan cystic macromolecules, paʻakikī loa.ʻO nā ʻano hana maʻamau o ka uhaʻi ʻana i nā paia cell bacteria e pili ana i ka ʻoki ʻana i ke kani ultrasonic, ka wili ʻana i ke one, ka extrusion kiʻekiʻe a i ʻole ka lāʻau lysozyme.Ma hope o ka haki ʻana o ka ʻiʻo a me nā pūnaewele, koho ʻia ka buffer kūpono e unuhi i ka protein i makemake ʻia.Wehe ʻia nā mea hiki ʻole ke hoʻoheheʻe ʻia e like me nā ʻāpana cellcentrifugeaiʻole kānana.

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