ʻO kapahu lulu erlenmeyerhe ipu moʻomeheu kūikawā no ka moʻomeheu cell suspension, a hiki ke hoʻohana ʻia e hoʻomākaukau, hui a mālama i nā ʻano media like ʻole.Hoʻohana ʻia kēia moʻomeheu i ka wā e kanu ai i nā pūnaewele Jurkat.

Loaʻa ka laina kelepona Jurkat mai ke koko ʻaoʻao o kahi keikikāne 14 makahiki a he cell hoʻomaha.Ua loaʻa mua nā laina kelepona i loaʻa iā Jurkat i nele i kekahi mau genes i nā waihona moʻomeheu cell.Hoʻohana nui ʻia nā laina T lymphocyte kanaka i hoʻopaʻa ʻia no ke aʻo ʻana i ka maʻi leukemia T cell acute, T cell signaling, a me nā mea hoʻokipa chemokine like ʻole i hiki ke komo i ka viral, ʻoi aku ka hōʻike HIV.He ākea loa ia o nā noi i ka noiʻi biological, e like me ka noi ʻana o nā pūnaewele Jurkat e aʻo i ka M1-RNA o ribonuclease P, a me ke aʻo ʻana i ka M1-RNA o ka anti-MHC class II transcriptional activator (CIITA) e kāohi. ka hōʻike ʻana o nā molekole MHC papa II ma ka ʻili pūnaewele.

I ka hoʻoulu ʻana i nā pūnaewele Jurkat i loko o nā pahu lulu erlenmeyer, RPMI1640 medium, 10% FBS pono;Hoʻomalu ka mahana ma 37 ° C, 5% carbon dioxide, PH waiwai 7.2-7.4, aseptic mau moʻomeheu mahana.Holoi a hoʻomaʻemaʻe me ka waiʻona 75% ma mua o ka hoʻololi ʻana i ke kahua hoʻomaʻemaʻe ultra-clean cell, e lawe i waho i ka cell cryovial mai ka pahu hauʻona wai, e hoʻokomo koke i loko o ka wai ʻauʻau 37 ° C a lulu i ka cryotube cell wikiwiki e hoʻoheheʻe koke ia.A laila, ma hope o ka centrifugation, pipetting and mixing, etc., ua waiho ʻia i loko o kahi incubator cell no ka mahi ʻana.

Pilikino loa nā kelepona i ke kaiapuni.I ka hoʻoulu ʻana i nā pūnaewele Jurkat i loko o nā pahu lulu erlenmeyer cell, pono e hana maikaʻi ʻia ka hoʻomaʻemaʻe pilikino, pono e hoʻohana i nā reagents sterile, a pono e hahai ʻia nā loina hana aseptic e pale aku i ka hoʻokomo ʻana i ka bacteria a pili i ka ulu ʻana o ka cell.