ʻO ka pahu hoʻoluliluli ʻo Erlenmeyer me ka pāpale puka makani
Hiʻona
1. E like me ka hana maʻamau c-GMP, ʻaʻohe pilina pilikino, kūpaʻa nui.
2. ʻO ka pāpale omole e hoʻohana ana i ka mea HDPE ikaika kiʻekiʻe a ua hoʻolālā ʻia me ka PTFE hydrophobic a me ka membrane hanu.
3. Akaka a pololei ka pālākiō, kahi kūpono no ka nānā ʻana i ka mana waena
4.ʻEhā mau mana o 125ml, 250ml, 500ml a me 1000ml
5. Aseptic paʻi kanaka
ʻO ka ʻokoʻa ma waena o ka pahu haʻalulu a me ka pahu erlenmeyer conical maʻamau
I ka wā o ka hoʻomohala wikiwiki ʻana o nā ʻenehana like ʻole, ua hoʻonui mau ʻia nā mea hoʻohana moʻomeheu cell a hoʻololi mau ʻia, a ʻo ka baffle shaker kahi moʻomeheu cell moʻomeheu hou.He aha nā ʻokoʻa ma waena o nā ipu maʻamau ʻelua?
ʻO ka mea mua, ma ke ʻano o ke ʻano, hoʻohana ʻia lāua ʻelua i kahi hoʻolālā triangular, a ua māhele ʻia nā ʻōmole i ʻelua mau ʻano: nā pāpale i hoʻopaʻa ʻia a me nā pāpale hanu, a ua like nā kikoʻī.ʻO ka ʻokoʻa nui ma waena o nā mea ʻelua ʻo ia ka lalo o ka ʻōmole.ʻO ka lalo o ka mea hoʻoluliluli maʻamau he pālahalaha, aʻo ka lalo o ka mea hoʻoluliluli he mau ʻāwī.ʻO nā ʻāpana i hoʻokiʻekiʻe ʻia o kēia mau ʻawaʻawa e hana i ka ʻōpala i loko o ka ʻōmole, no laila inoa.
ʻElua mau hana o ka hoʻolālā kūikawā o ka pahu baffle.ʻO kekahi e hōʻemi i ke ʻano o ka clumping cell.Hiki i ka lulu ʻana me ka lulu ke hoʻemi pono i ka viscosity i hoʻokumu ʻia e ka DNA manuahi a me nā ʻōpala cell, a hoʻemi i ka ulu ʻana o ka ulu ʻana o ka cell clumping.Eia kekahi, hiki i ka baffle ma lalo ke pale aku i ka hanana vortex i hana ʻia e ka medium i ka wā e haʻalulu ai, e hoʻonui ai i ke ʻano like ʻole, a he hopena kekahi i ka hōʻemi ʻana i ka clumping cell.ʻO ka lua e hoʻonui i ka nui o ka oxygen dissolved.Hiki i ka baffle ma lalo o ka hue ke hoʻonui i ka nui o ka oxygen i hoʻoheheʻe ʻia i ka waena, hoʻoikaika maikaʻi i ka pilina piha ma waena o nā cell a me ka ea, a kōkua i nā cell e ulu maikaʻi.
Ma keʻano laulā, ʻo ka ʻokoʻa nui ma waena o nā pahu lulu baffle a meipu lulu maʻamauʻo ka ʻokoʻa ma lalo o ka ʻōmole.ʻO ke ʻano o ka ʻōmole hou e hoʻonui i ka nui o ka oxygen dissolved a ʻoi aku ka maikaʻi no nā laina kelepona e pono ai ka nui o ka oxygen.
ʻElua mau hiʻohiʻona o ka pahu erlenmeyer hoʻohāhā
1. E ho'ēmi i ka clumping pūnaewele
I ke kaʻina o ka moʻomeheu cell suspension, ʻike pinepine ʻia ka ulu ʻana o ka cell clumping.He ʻokoʻa nā kumu, e like me ka nele o ka hoʻokuʻu hou ʻana ma hope o ka centrifugation, a i ʻole ka pilikia o ka serum i ka waena, a i ʻole ka hoʻopaʻa ʻana o nā ion calcium a me ka magnesium.hoʻololi i ka adhesion ma waena o nā pūnaewele.Hoʻoluliluli ʻia ka pahu baffle me kahi haʻalulu, hiki ke hōʻemi pono i ka viscosity i hoʻokumu ʻia e ka DNA manuahi a me ka ʻōpala cell, a hoʻemi i ka ulu ʻana o ka ulu ʻana o ka cell clumping.Eia kekahi, hiki i ka baffle ma lalo ke pale i ka hanana vortex i hoʻokumu ʻia e ka medium i ka wā e haʻalulu ai, e hoʻonui ai i ke ʻano like ʻole, e hōʻemi ana i ka manawa o ka puʻupuʻu cell i kekahi ʻano.
2. Hoʻonui i ka oxygen i hoʻoheheʻe ʻia
ʻO ka pāpale hanu he ala koʻikoʻi no ka hoʻololi kinoea o ka ʻōmole haʻalulu.Ma o ka hana hanu o ka membrane hanu, ma kekahi ʻaoʻao, hiki iā ia ke hoʻoikaika i ka hoʻololi kinoea i loko o ka hue, a ma ka ʻaoʻao ʻē aʻe, hiki iā ia ke pale pono i ka haumia o nā microorganisms.Hiki i ka baffle ma lalo o ka hue ke hoʻonui i ka nui o ka oxygen dissolved i ka moʻomeheu moʻomeheu, e hoʻoikaika pono i ka pilina ma waena o nā cell a me ka ea, a laila e hoʻomaikaʻi ai i ka maikaʻi o ka hoʻololi ʻana i ke kinoea a hiki i nā cell ke ulu maikaʻi.
ʻO ka hoʻolālā kūikawā o ka baffle flask ma muli o nā ʻōpala ma lalo o ka ʻōmole, e hōʻemi ana i ka clumping cell, hoʻonui i ka oxygen dissolved, a hāʻawi i nā kūlana maikaʻi no ka ulu ʻana o ka cell.
● Lahui Huahana
Māhele | Helu ʻatikala | Volume | Kāpena | Mea waiwai | Hōʻike pūʻolo | Ka nui pahu |
ʻO ka pahu Erlenmeyer pōʻalo, PETG | LR036125 | 125ml | sila Cap | PETG,ʻO ka sterilization iradiation | 1 mau ʻāpana/pack24pack/case | 31 X 21 X 22 |
LR036250 | 250ml | 1 mau ʻeke / ʻeke12 ʻeke / pahu | 31 X 21 X 22 | |||
LR036500 | 500ml | 1 mau ʻeke / ʻeke12 ʻeke / pahu | 43 X 32 X 22 | |||
LR036001 | 1000ml | 1 mau ʻeke / ʻeke12 ʻeke / pahu | 55 X 33.7 X 24.5 | |||
ʻO ka pahu Erlenmeyer pōʻalo, PETG | LR037125 | 125ml | Hoʻopaʻa hoʻoheheʻe | PETG,ʻO ka sterilization iradiation | 1 mau ʻāpana/pack24pack/case | 31 X 21 X 22 |
LR037250 | 250ml | 1 mau ʻeke / ʻeke12 ʻeke / pahu | 31 X 21 X 22 | |||
LR037500 | 500ml | 1 mau ʻeke / ʻeke12 ʻeke / pahu | 43 X 32 X 22 | |||
LR037001 | 1000ml | 1 mau ʻeke / ʻeke12 ʻeke / pahu | 55 X 33.7 X 24.5 | |||
Puke Erlenmeyer pohihihi, PC | LR034125 | 125ml | sila Cap | PC, hoʻomaʻamaʻa ʻana i ka iradiation | 1 mau ʻāpana/pack24pack/case | 31 X 21 X 22 |
LR034250 | 250ml | 1 mau ʻeke / ʻeke12 ʻeke / pahu | 31 X 21 X 22 | |||
LR034500 | 500ml | 1 mau ʻeke / ʻeke12 ʻeke / pahu | 43 X 32 X 22 | |||
LR034001 | 1000ml | 1 mau ʻeke / ʻeke12 ʻeke / pahu | 55 X 33.7 X 24.5 | |||
Puke Erlenmeyer pohihihi, PC | LR035125 | 125ml | Hoʻopaʻa hoʻoheheʻe | PC, hoʻomaʻamaʻa ʻana i ka iradiation | 1 mau ʻāpana/pack24pack/case | 31 X 21 X 22 |
LR035250 | 250ml | 1 mau ʻeke / ʻeke12 ʻeke / pahu | 31 X 21 X 22 | |||
LR035500 | 500ml | 1 mau ʻeke / ʻeke12 ʻeke / pahu | 43 X 32 X 22 | |||
LR035001 | 1000ml | 1 mau ʻeke / ʻeke12 ʻeke / pahu | 55 X 33.7 X 24.5 |