ʻO Fluorescence Quantitative PCR Detection 96 mau laʻana
Ma ke ʻano he koho kūpono no ka loiloi nui o ka biology molecular, ua hoʻohana nui ʻia ka ʻōnaehana PCR manawa maoli i nā ʻano like ʻole e like me ka noiʻi ʻepekema, ka ʻike ʻana a me ka maʻi maʻi, ka maikaʻi a me ka palekana palekana, a me nā noi forensic.
Pūnaehana PCR manawa maoli
Pololei 96
Nā hiʻohiʻona
• A hiki i ka 6 kaila ʻike fluorescence e ʻae ana i ka PCR multiplex.
• Hoʻemi maikaʻi i ka crosstalk multi-color a me ka hopena lihi, ʻaʻohe hoʻoponopono ROX pono e hōʻemi i ka hoʻohana ʻana i ka laʻana a me ka reagent.
• ʻO ke kaʻina hana scan hou a me ka ʻenehana hoʻokaʻawale hōʻailona i hoʻoholo ʻia e hoʻomaikaʻi i ka ʻike ʻike
• ʻenehana uku wela lihi kū hoʻokahi e hōʻemi i ka "hopena lihi"
• mea hoʻohana-aloha lako polokalamu
• ʻO kaʻenehana hou me ka kukui LED lōʻihi e hāʻawi i nā hopena hilinaʻi
Nā ʻāpana ʻenehana
Pūnaehana hoʻomalu mahana | |
Kaha laʻana | 96 |
Ka nui o ka pane | 10-50 μl |
ʻenehana pōʻai wela | Peltier |
Max.Laki hoʻomehana/hoʻolili | 6.0° C/s |
Laulā wela wela | 4 – 100 °C |
Pono ka wela | ± 0.2°C |
Kaulike mahana | ±0.2 ℃ @60 ℃ , ± 0.3 ℃ @95 ℃ |
Laulā hoʻonohonoho gradient wela | 30–100°C |
Laulā hoʻonohonoho ʻokoʻa ʻokoʻa wela | 1 – 36°C |
Pūnaehana ʻike | |
Puna kukui hoʻohauʻoli | 4/6 monochrome kiʻekiʻe pono LED |
Mea ʻike maka | PMT |
ʻAno ʻike | ʻenehana hoʻokaʻawale hōʻailona manawa |
ʻO ka laulā o ka lōʻihi o ka nalu hauʻoli | 455-650nm/510-715nm |
ʻO nā ʻauwaʻa fluorescent | 4/6 kaha |
Kākoʻo kala | FAM/SYBR ʻōmaʻomaʻo, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas ʻulaʻula, Mustang Poni, Cy5/LIZ |
ʻO ka ʻike | Gene kope hoʻokahi |
Olelo Hooholo | Hiki ke ʻike ʻia ka ʻokoʻa helu kope 1.33 i loko o ka qPCR hoʻokahi-plex |
Laina lohi | 10 kauoha o ka nui kope |